製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to GPCR GPR81
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-QQLARQARMKKATR

    , corresponding to internal sequence amino acids 207-220 of Human GPCR GPR81 (NP_115943.1).

  • ポジティブ・コントロール
    • Human liver lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa).

ターゲット情報

  • 機能
    Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.
  • 組織特異性
    Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
  • 別名
    • FKSG80 antibody
    • FKSG80 protein antibody
    • G protein coupled receptor 104 antibody
    • G protein coupled receptor 81 antibody
    • G protein coupled receptor GPR81 antibody
    • G-protein coupled receptor 104 antibody
    • G-protein coupled receptor 81 antibody
    • GPR 104 antibody
    • GPR 81 antibody
    • GPR104 antibody
    • GPR81 antibody
    • GPR81_HUMAN antibody
    • HCA1 antibody
    • HCAR1 antibody
    • hydroxy-carboxylic acid receptor 1 antibody
    • Hydroxycarboxylic acid receptor 1 antibody
    • LACR1 antibody
    • lactate receptor 1 antibody
    • Putative chemokine receptor antibody
    • T cell activation G protein coupled receptor antibody
    • TA GPCR antibody
    see all

画像

  • Anti-GPCR GPR81 antibody (ab106942) at 0.5 µg/ml + Human liver lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 hourAn additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.

プロトコール

参考文献

This product has been referenced in:
  • Wagner W  et al. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal 13:36 (2015). WB ; Human . Read more (PubMed: 26208712) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab106942.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録