製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR78
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 261-363 of Human GPCR GPR78.

  • ポジティブ・コントロール
    • Human pancreas tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval at pH6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP.
  • 組織特異性
    High level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ98800 antibody
    • G protein coupled receptor GPR78 antibody
    • G-protein coupled receptor 78 antibody
    • GPR78 antibody
    • GPR78_HUMAN antibody
    • PRO19818 antibody
    • UNQ5925 antibody
    see all

画像

  • ab121390, at 1/25 dilution, staining GPCR GPR78 in paraffin-embedded Human pancreas tissue by Immunohistochemistry, showing distinct cytoplasmic and membranous positivity in intercalated ducts.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

参考文献

This product has been referenced in:
  • Dong DD  et al. GPR78 promotes lung cancer cell migration and metastasis by activation of Gaq-Rho GTPase pathway. BMB Rep 49:623-628 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27697106) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab121390.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録