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RabMAb

Anti-GPCR GPR49 抗体 [EPR3065Y] (ab75850)

製品の概要

  • 製品名
    Anti-GPCR GPR49 antibody [EPR3065Y]
    GPCR GPR49 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3065Y] to GPCR GPR49
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GPCR GPR49 aa 800-900 (C terminal).

  • ポジティブ・コントロール
    • Fetal skeletal muscle lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 100 kDa.
IP 1/20.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • ターゲット情報

    • 機能
      Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
    • 組織特異性
      Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
    • 配列類似性
      Belongs to the G-protein coupled receptor 1 family.
      Contains 16 LRR (leucine-rich) repeats.
      Contains 1 LRRNT domain.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FEX antibody
      • G protein coupled receptor 49 antibody
      • G protein coupled receptor 67 antibody
      • g protein-coupled receptor fex antibody
      • G-protein coupled receptor 49 antibody
      • G-protein coupled receptor 67 antibody
      • G-protein coupled receptor HG38 antibody
      • GPR 49 antibody
      • GPR 67 antibody
      • GPR49 antibody
      • GPR67 antibody
      • GRP 49 antibody
      • GRP49 antibody
      • HG 38 antibody
      • HG38 antibody
      • Leucine rich repeat containing G protein coupled receptor 5 antibody
      • Leucine-rich repeat-containing G-protein coupled receptor 5 antibody
      • LGR 5 antibody
      • LGR5 antibody
      • LGR5_HUMAN antibody
      • MGC117008 antibody
      • Orphan G protein coupled receptor HG38 antibody
      see all

    Anti-GPCR GPR49 antibody [EPR3065Y] 画像

    • Anti-GPCR GPR49 antibody [EPR3065Y] (ab75850) at 1/1000 dilution + Fetal skeletal muscle lysate at 10 µg

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 100 kDa

    Anti-GPCR GPR49 antibody [EPR3065Y] (ab75850) 使用論文

    This product has been referenced in:
    • Kantara C  et al. Methods for detecting circulating cancer stem cells (CCSCs) as a novel approach for diagnosis of colon cancer relapse/metastasis. Lab Invest 95:100-12 (2015). Read more (PubMed: 25347154) »
    • Jardé T  et al. ERBB3 Positively Correlates with Intestinal Stem Cell Markers but Marks a Distinct Non Proliferative Cell Population in Colorectal Cancer. PLoS One 10:e0138336 (2015). ICC/IF ; Human . Read more (PubMed: 26367378) »

    See all 16 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - BSA
    Specification
    Colon
    Blocking step
    Hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Username

    Vinicius Kannen Cardoso

    Verified customer

    投稿 Sep 09 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Ahmar Aziz

    Verified customer

    投稿 Dec 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    none
    Permeabilization
    No
    Sample
    Mouse Cell (colon)
    Specification
    colon
    Gating Strategy
    High FSC/SSC log scale
    Preparation
    Cell harvesting/tissue preparation method: isolate colon, collagenase digestion
    Sample buffer: DMEM
    Username

    Abcam user community

    Verified customer

    投稿 Aug 20 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SW620 Colon)
    Loading amount
    100 µg
    Specification
    SW620 Colon
    Treatment
    siRNA - knockdown
    Gel Running Conditions
    Reduced Denaturing (7)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 08 2013


    Protein G beads were indeed used with ab75850 following this protocol for IP.


    I have contacted the lab and ab75850 tested positive in IP (image attached). Rat heart is the only sample we have tested. Our recommended IP protocol is described at the following link: http://www.epitomics.com/products/support/ip, and is the same...

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
    Permeabilization
    No
    Specification
    Intestine
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Feb 12 2013

    Thank you for your enquiry.

    I can confirm that ab75850 Anti-GPCR GPR49 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a con...

    Read More

    Thank you for your reply.

    I have gone ahead and processed your request for a free of charge replacement antibody and you should have it by 10.30am tomorrow morning. Your new order number is 1126740.

    If there is anything else I can h...

    Read More

    Thank you for providing that information.

    Do you wish to recieve another vial of ab75850, or do wish to reciece a different antibody instead? Once I hear back from you, I will arrange the repalcement antibody to be sent as soon as possible. <...

    Read More

    1-10 of 23 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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