製品の概要

  • 製品名Anti-GPCR GPR43 antibody
    GPCR GPR43 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR43
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region from the 2nd extracellular domain of Human GPCR GPR43.

  • ポジティブ・コントロール
    • Human Lung, Alveolar Macrophages tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 35 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Receptor for short chain fatty acids through a G(i)-protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate.
  • 組織特異性Expressed at relatively high levels in peripheral blood leukocytes and, to lesser, extent, in spleen.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FFA2R antibody
    • Ffar2 antibody
    • FFAR2_HUMAN antibody
    • free fatty acid activated receptor 2 antibody
    • Free fatty acid receptor 2 antibody
    • G protein coupled receptor 43 antibody
    • G protein-coupled receptor 43 antibody
    • G-protein coupled receptor 43 antibody
    • GPR 43 antibody
    • GPR43 antibody
    see all

Anti-GPCR GPR43 antibody 画像

  • Human Lung, Alveolar Macrophages (formalin-fixed, paraffin-embedded) stained with ab118449 at 35 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab118449 staining GPCR GPR43 in 4µm parasagittal FFPE sections from adult mouse brain and spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation, pH6.0. Tissue was blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in 1M PBS + 5% BSA) for 24 hours at 4°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (1/500, 2 hours at 20°C) was used as the secondary antibody. Second sections were incubated with the anti-Iba-1 antibody for 24 h (dilution 1/500 at 4 °C), followed by the Alexa Fluor® 568-conjugated secondary antibody staining (1/500 for 2 hours at 20°C). Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI).

    See Abreview

Anti-GPCR GPR43 antibody (ab118449) 使用論文

This product has been referenced in:
  • Erny D  et al. Host microbiota constantly control maturation and function of microglia in the CNS. Nat Neurosci N/A:N/A (2015). IHC-P ; Mouse . Read more (PubMed: 26030851) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9 (ER2) 20 mins
Permeabilization No
Specification Liver
Blocking step h2o2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Formaldehyde
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投稿 Nov 29 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain, spleen)
Antigen retrieval step Heat mediated
Permeabilization No
Specification brain, spleen
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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投稿 Jun 05 2015



Anti-GPCR GPR43 antibody was raised against a synthetic 17 amino acid peptide from 2nd extracellular domain of human GPR43. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (94%); Mouse, Rat, Dog, Panda, Ra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"