製品の概要

  • 製品名Anti-GPCR GPR18 antibody
    GPCR GPR18 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR18
  • アプリケーション適用あり: WB, ELISA, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthesized peptide derived from the internal region of human GPCR GPR18

  • ポジティブ・コントロール
    • Hela cells. Extracts from HT-29 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
Flow Cyt 1/100 - 1/400. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性G protein coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR18 is a member of this family and has been reported to be expressed at high levels in testis and spleen and at lower levels in other tissues associated with endocrine and immunologic/hematologic functions. It is thought to be a receptor for N arachidonyl glycine. It may contribute to regulation of the immune system.
  • 細胞内局在Cell membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • G protein coupled receptor 18 antibody
    • GPCRW antibody
    • GPR 18 antibody
    • GPR18 antibody
    • N arachidonyl glycine receptor antibody
    • NAGly receptor antibody
    see all

Anti-GPCR GPR18 antibody 画像

  • All lanes : Anti-GPCR GPR18 antibody (ab76258) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from HT-29 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 117 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells using ab76258 at a 1/500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • ab76258 detecting GPCR GPR18 in Human synovial fibroblasts by Flow Cytometry. Cells were detached with accutase (PAA), fixed with formaldehyde and permeabilized with methanol (15 minutes, -20°C). Cells were gated on fibroblasts and incubated with the primary antibody diluted 1/400 (PBS, 1% BSA) and incubated with the sample for 30 minutes at 21°C. The secondary was a biotin-conjugated Goat anti-Rabbit polyclonal antibody diluted 1/168. Detected with streptavidin/PE 0.003µg/ml (1% BSA in PBS, 30 minutes). The red histogram indicates the isotype control, black histogram is the specific stain for GPCR GPR18.

    See Abreview

Anti-GPCR GPR18 antibody (ab76258) 使用論文

This product has been referenced in:
  • Wilhelmsen K  et al. The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells. J Biol Chem 289:13079-100 (2014). WB ; Human . Read more (PubMed: 24644287) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (synovial fibroblasts)
Specification synovial fibroblasts
Fixative Formaldehyde
Permeabilization Yes - 0,1% TritonX-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Dr. Torsten Lowin

Verified customer

投稿 Dec 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (synovial fibroblasts)
Specification synovial fibroblasts
Preparation Cell harvesting/tissue preparation method: cells detached with accutase (PAA)
Sample buffer: PBS with 1%BSA
Fixation Formaldehyde
Permeabilization Yes - methanol, 15min -20 degrees
Gating Strategy only one gate: fibroblasts
Username

Dr. Torsten Lowin

Verified customer

投稿 Nov 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"