Anti-GPCR GPR18 抗体 (ab76258)
Key features and details
- Rabbit polyclonal to GPCR GPR18
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GPCR GPR18 antibody
GPCR GPR18 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GPCR GPR18 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
A synthesized peptide derived from the internal region of human GPCR GPR18
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76258の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 38 kDa.
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ICC/IF | (1) |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 38 kDa. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system. -
組織特異性
Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2841 Human
- Omim: 602042 Human
- SwissProt: Q14330 Human
- Unigene: 741589 Human
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別名
- G protein coupled receptor 18 antibody
- G-protein coupled receptor 18 antibody
- GPCRW antibody
see all
画像
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All lanes : Anti-GPCR GPR18 antibody (ab76258) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells
Lane 2 : Extracts from HT-29 cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 117 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of HeLa cells using ab76258 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab76258 は 3 報の論文で使用されています。
- Jundi B et al. Inflammation resolution circuits are uncoupled in acute sepsis and correlate with clinical severity. JCI Insight 6:N/A (2021). PubMed: 34166226
- Ulu A et al. Omega-3 Fatty Acid-Derived Resolvin D2 Regulates Human Placental Vascular Smooth Muscle and Extravillous Trophoblast Activities. Int J Mol Sci 20:N/A (2019). PubMed: 31500240
- Wilhelmsen K et al. The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells. J Biol Chem 289:13079-100 (2014). WB ; Human . PubMed: 24644287