製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR15
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Peptide corresponding to amino acids 13 to 28 of human GPR15 (1). The sequence differs from those ofAfrican green monkey and pig-tailed macaque BOB byone amino acid (2)

  • 特記事項
    Stable for one year.


    Human immunodeficiency virus (HIV) and related viruses require co-receptors to infect target cells. Some G protein coupled receptors including CCR5, CXCR4, CCR3,CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively) were recently cloned. This novel G protein-coupled receptor serves as coreceptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 has not been identified yet.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    Human immunodeficiency virus (HIV) and related viruses require co-receptors to infect target cells. Some G protein coupled receptors including CCR5, CXCR4, CCR3,CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively) were recently cloned. This novel G protein-coupled receptor serves as coreceptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 has not been identified yet.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 50 kDa.Can be blocked with GPCR GPR15 peptide (ab6101).

ターゲット情報

画像

  • All lanes : Anti-GPCR GPR15 antibody (ab8104)

    Lane 1 : Human heart lysate in absence of specific peptide
    Lane 2 : Human heart lysate in presence of specific peptide
    Lane 3 : Human heart lysate in presence of unrelated peptide


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Lane 1 : Anti-GPCR GPR15 antibody (ab8104) at 0.5 µg/ml
    Lane 2 : Anti-GPCR GPR15 antibody (ab8104) at 1 µg/ml

    Lane 1 : Human spleen lysate
    Lane 2 : Human spleen lysate


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Pan B  et al. G-protein coupled receptor 15 mediates angiogenesis and cytoprotective function of thrombomodulin. Sci Rep 7:692 (2017). WB . Read more (PubMed: 28386128) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Caroline Schmutz

Verified customer

投稿 Dec 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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