製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR15
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide:

    YYATSPNSDIRETHSH

    , corresponding to N terminal amino acids 13-28 of Human GPCR GPR15.

  • ポジティブ・コントロール
    • Jurkat, A549 or K562 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 40 kDa.Can be blocked with GPCR GPR15 peptide (ab6101). Detects a band of approximately 50 kDa in human heart tissue lysate.

ターゲット情報

プロトコール

Anti-GPCR GPR15 antibody (ab16445) 使用論文

ab16445 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH-3T3 cell line)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Rippa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
NIH-3T3 cell line
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 15 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cytospined HEK293 human cells)
Permeabilization
No
Specification
Cytospined HEK293 human cells
Fixative
Any
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 13 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
THP-1, A549, HEK293, CEMx-174
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 09 2015

Application
Flow Cytometry
Fixation
None, cells were used fresh
Permeabilization
No
Sample
Mouse Cell (Cell from bone marrow)
Specification
Cell from bone marrow
Gating Strategy
Alive cells (DAPI-), blood cells excluded
Preparation
Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

投稿 Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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