製品の概要

  • 製品名Anti-GPCR GPR15 antibody
    GPCR GPR15 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR15
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    YYATSPNSDIRETHSH

    , corresponding to N terminal amino acids 13-28 of Human GPCR GPR15.

  • ポジティブ・コントロール
    • Jurkat, A549 or K562 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 40 kDa.Can be blocked with GPCR GPR15 peptide (ab6101). Detects a band of approximately 50 kDa in human heart tissue lysate.

ターゲット情報

Anti-GPCR GPR15 antibody (ab16445) 使用論文

ab16445 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3 cell line)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 5 µg
Treatment Cells were mixed in Rippa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification NIH-3T3 cell line
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 15 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Cytospined HEK293 human cells)
Permeabilization No
Specification Cytospined HEK293 human cells
Fixative Any
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 13 2015

Application Western blot
Sample Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 5 µg
Treatment Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification THP-1, A549, HEK293, CEMx-174
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 09 2015

Application Flow Cytometry
Fixation None, cells were used fresh
Permeabilization No
Sample Mouse Cell (Cell from bone marrow)
Specification Cell from bone marrow
Gating Strategy Alive cells (DAPI-), blood cells excluded
Preparation Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

投稿 Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"