製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR126
  • 特異性
    Detects endogenous levels of total GPCR GPR126 protein.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from an internal region of human GPCR GPR126

  • ポジティブ・コントロール
    • Extracts from HUVEC cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能
    Orphan receptor. May be required for normal differentiation of promyelinating Schwann cells and for normal myelination of axons. Signals probably through G-proteins to transiently elevate cAMP levels.
  • 組織特異性
    Expressed in placenta and to a lower extent in pancreas and liver. Detected in aortic endothelial cells but not in skin microvascular endothelial cells.
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 CUB domain.
    Contains 1 GPS domain.
    Contains 1 pentaxin domain.
  • 翻訳後修飾
    Proteolytically cleaved into 2 conserved sites: one in the GPS domain (S1 site) and the other in the middle of the extracellular domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. Furin is involved in the cleavage of the S2 site generating a soluble fragment. Processing at the GPS domain occurred independent of and probably prior to the cleavage at the S2 site.
  • 細胞内局在
    Cell membrane. Detected on the cell surface of activated but not resting umbilical vein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • adhesion G protein coupled receptor G6 antibody
    • APG1 antibody
    • Developmentally regulated G protein coupled receptor antibody
    • Developmentally regulated G-protein-coupled receptor antibody
    • dj287g14.2 antibody
    • DREG antibody
    • G protein coupled receptor 126 antibody
    • G-protein coupled receptor 126 antibody
    • GP126_HUMAN antibody
    • GPR126 antibody
    • HBV PreS1 transactivated protein 2 antibody
    • HBV PreS1-transactivated protein 2 antibody
    • LCCS9 antibody
    • OTTHUMP00000017320 antibody
    • OTTHUMP00000237093 antibody
    • OTTHUMP00000237094 antibody
    • OTTHUMP00000237115 antibody
    • PR126 antibody
    • Probable G protein coupled receptor 126 [Precursor] antibody
    • Probable G protein coupled receptor 126 antibody
    • Probable G-protein coupled receptor 126 [Precursor] antibody
    • Probable G-protein coupled receptor 126 antibody
    • PS1TP2 antibody
    • Vascular inducible G protein coupled receptor antibody
    • Vascular inducible G protein-coupled receptor antibody
    • VIGR antibody
    see all

画像

  • All lanes : Anti-GPCR GPR126 antibody (ab75356) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 137 kDa
    Observed band size : 137 kDa

プロトコール

参考文献

This product has been referenced in:
  • Kitagaki J  et al. A Putative Association of a Single Nucleotide Polymorphism in GPR126 with Aggressive Periodontitis in a Japanese Population. PLoS One 11:e0160765 (2016). WB . Read more (PubMed: 27509131) »
  • Cui H  et al. GPR126 protein regulates developmental and pathological angiogenesis through modulation of VEGFR2 receptor signaling. J Biol Chem 289:34871-85 (2014). Read more (PubMed: 25217645) »

See all 2 Publications for this product

レビューと Q&A


The antibody has not been predicted to cross react with mouse GPR126. The lab aligned the exact immunogenic sequence used to raise the antibody with Q6F3F9, and the homology is only 64.3%.

We usually say that a homology of 85% or higher h...

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Thank you for this feedback. I am sorry that I could not be of more help on this occasion. If you like to get some more information on our testing discount program, please find the Terms and Conditions of this offer at: www.abcam.com/collabora...

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Thank you for contacting us. I am happy to let you know the following immunogen sequences: ab65175: xxxxxxxxxxxxxxxxxx ab75356: yyyyyyyyyyyyyyyy In preliminary searches, I could not find conclusive matches for these peptides in zebrafish (onl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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