製品の概要

  • 製品名Anti-GPCR GPR101 antibody
    GPCR GPR101 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GPCR GPR101
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized peptide derived from C-terminal region of human GPCR GPR101.

  • ポジティブ・コントロール
    • Extracts from COS-7 cells; HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

Anti-GPCR GPR101 antibody 画像

  • All lanes : Anti-GPCR GPR101 antibody (ab75241) at 1/500 dilution

    Lane 1 : extracts from COS-7 cells
    Lane 2 : extracts from COS-7 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75241. Right panel: same, after preincubation with the immunizing peptide.
  • IHC image of ab75241 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75241, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GPCR GPR101 antibody (ab75241) 使用論文

ab75241 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"