The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Receptor for an unknown ligand. Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May serve as a mechanism for T- and B-cells, and other cell types, to slow their proliferation and repair damaged DNA to ensure proper replication.
Highly expressed in macrophages and hematopoietic tissues rich in lymphocytes, like spleen and thymus. Weakly expressed in heart and lung. In atherosclerotic plaques, expression is observed around the lipid core and at the shoulder region.
Belongs to the G-protein coupled receptor 1 family.
Cell membrane. Internalized and accumulated in endosomal compartments. LPC triggers the relocalization from the endosomal compartment to the cell surface.
Immunohistochemistry Image of Human Spleen (formalin-fixed, paraffin-embedded) stained with GPCRG2A antibody (ab116586)at 11 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.