製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GPBP1
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide (Human). More details of the immunogen are not available.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab42457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-GPBP1 antibody (ab42457) at 0.5 µg/ml + HepG2 Cell Lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/75000 dilution

    Predicted band size : 53 kDa
    Observed band size : 52,59 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 59 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Paraffin-embedded Human Kidney sections) using ab42457 at 4-8 ug/ml. Positively labelled epithelial cells of tubule are indicated using arrows.

参考文献

ab42457 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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