製品の概要
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製品名Anti-GPBP1 antibody
GPBP1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to GPBP1
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog
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免疫原
Synthetic peptide within Human GPBP1 aa 67-116 (N terminal). The exact sequence is proprietary.
Sequence:RKEKNGWRTHGRNGTENINHRGGYHGGSSRSRSSIFHAGKSQGLHENNIP
Database link: Q86WP2
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab42457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Functions as a GC-rich promoter-specific transactivating transcription factor.
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組織特異性Widely expressed. Some isoforms may be specifically expressed in veins and arteries (at protein level). Isoform 4 is widely expressed. Isoform 1, isoform 2 and isoform 3 may be specifically expressed in vascular smooth muscle cells.
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配列類似性Belongs to the vasculin family.
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細胞内局在Nucleus. Cytoplasm. According to PubMed:12842993, it localizes to the cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 613751 Cow
- Entrez Gene: 65056 Human
- Entrez Gene: 73274 Mouse
- Entrez Gene: 294734 Rat
- SwissProt: Q0P5K1 Cow
- SwissProt: Q86WP2 Human
- SwissProt: Q9H0D4 Human
- SwissProt: Q9H785 Human
see all -
別名
- DKFZp761C169 antibody
- GC rich promoter binding protein 1 antibody
- GC-rich promoter-binding protein 1 antibody
see all
画像
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Western blot - GPBP1 antibody (ab42457)Anti-GPBP1 antibody (ab42457) at 0.5 µg/ml + HepG2 Cell Lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/75000 dilution
Predicted band size: 53 kDa
Observed band size: 52,59 kDa (why is the actual band size different from the predicted?)
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Paraffin-embedded sections) - GPBP1 antibody (ab42457)Immunohistochemistry (Paraffin-embedded Human Kidney sections) using ab42457 at 4-8 ug/ml. Positively labelled epithelial cells of tubule are indicated using arrows.
参考文献
ab42457 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"