製品の概要

  • 製品名Anti-GPBB antibody [17B6]
    GPBB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [17B6] to GPBB
  • 特異性This product reacts with BB and MM isoenzyme.
  • アプリケーション適用あり: ICC/IF, WB, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human).

  • 特記事項Concentration varies from lot to lot and can be provided on request.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
AP Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be used as Capture antibody. Can be paired for ELISA with Mouse monoclonal [10H5] to GPBB.

ターゲット情報

  • 機能Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • 配列類似性Belongs to the glycogen phosphorylase family.
  • 翻訳後修飾Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brain glycogen phosphorylase antibody
    • Glycogen phosphorylase B antibody
    • Glycogen phosphorylase brain form antibody
    • Glycogen Phosphorylase Isoenzyme BB antibody
    • Glycogen phosphorylase, brain form antibody
    • MGC9213 antibody
    • Phosphorylase glycogen brain antibody
    • PYGB antibody
    • PYGB_HUMAN antibody
    see all

Anti-GPBB antibody [17B6] 画像

  • ICC/IF image of ab880 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab880, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GPBB antibody [17B6] (ab880) 使用論文

ab880 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"