製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to GPAM
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length GPAM (Human). (AAH30783.1).

  • ポジティブ・コントロール
    • GPAM transfected 293T cell lysate.

アプリケーション

Our Abpromise guarantee covers the use of ab68925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 94 kDa.

ターゲット情報

  • 機能
    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
  • パスウェイ
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
  • 配列類似性
    Belongs to the GPAT/DAPAT family.
  • ドメイン
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • 細胞内局在
    Mitochondrion outer membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glycerol 3 phosphate acyltransferase 1 mitochondrial antibody
    • Glycerol 3 phosphate acyltransferase mitochondrial antibody
    • Glycerol-3-phosphate acyltransferase 1 antibody
    • Gpam antibody
    • GPAT antibody
    • GPAT-1 antibody
    • GPAT1 antibody
    • GPAT1_HUMAN antibody
    • KIAA1560 antibody
    • MGC26846 antibody
    • mitochondrial antibody
    • RP11-426E5.2 antibody
    see all

画像

  • All lanes : Anti-GPAM antibody (ab68925) at 1/500 dilution

    Lane 1 : GPAM transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 94 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Zhang C  et al. Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling. Proc Natl Acad Sci U S A 109:1667-72 (2012). Read more (PubMed: 22307628) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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