保存方法 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
精製度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
機能 Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
組織特異性 Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues.
パスウェイ Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
細胞内局在 Endoplasmic reticulum membrane.
Information by UniProt
Anchor attachment protein 1 (Gaa1p, yeast) homolog antibody
GAA1 protein homolog antibody
Anti-GPAA1 antibody 画像
Western blot - GPAA1 antibody (ab66032)
Anti-GPAA1 antibody (ab66032) at 0.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer Secondary HRP conjugated anti-rabbit IgG at 50,000 - 100,000 dilution Predicted band size : 68 kDa Observed band size : >70 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GPAA1 antibody(ab66032)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPAA1 with ab66032 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
Anti-GPAA1 antibody (ab66032) 使用論文
has not yet been referenced specifically in any publications.
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