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RabMAb

Anti-GPA33 抗体 [EPR4240] (ab108938)

製品の概要

  • 製品名
    Anti-GPA33 antibody [EPR4240]
    GPA33 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4240] to GPA33
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human GPA33. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HT-29, Human small intestine, and Human fetal colon lysates. Human colon tissue. Human colonic adenocarcinoma tissue.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      May play a role in cell-cell recognition and signaling.
    • 組織特異性
      Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
    • 配列類似性
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻訳後修飾
      N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
      Palmitoylated.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A33 antibody
      • Cell surface A33 antigen antibody
      • Cell surface A33 antigen precursor antibody
      • Glycoprotein A33 (transmembrane) antibody
      • Glycoprotein A33 antibody
      • Gpa33 antibody
      • GPA33_HUMAN antibody
      • MGC129986 antibody
      • MGC129987 antibody
      • OTTHUMP00000032346 antibody
      • Transmembrane glycoprotein A33 antibody
      see all

    画像

    • All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution

      Lane 1 : HT-29 lysate
      Lane 2 : Human small intestine lysate
      Lane 3 : Human fetal colon lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108938 showing negative staining in Breast carcinoma tissue.

    • ab108938 showing negative staining in Thyroid gland carcinoma tissue.

    • ab108938 showing positive staining in Ovarian carcinoma tissue.

    • ab108938 showing negative staining in Normal tonsil tissue.

    • ab108938 showing negative staining in Normal liver tissue.

    参考文献

    This product has been referenced in:
    • Gotanda K  et al. Circulating intestine-derived exosomal miR-328 in plasma, a possible biomarker for estimating BCRP function in the human intestines. Sci Rep 6:32299 (2016). WB, IP . Read more (PubMed: 27571936) »

    See 1 Publication for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - other (Colorectal Cancer Cell Lines and Exosomes)
    Gel Running Conditions
    Reduced Denaturing (4- 20%)
    Loading amount
    40 µg
    Specification
    Colorectal Cancer Cell Lines and Exosomes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Fabia Fricke

    Verified customer

    投稿 Dec 19 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrat
    Permeabilization
    No
    Specification
    Colon tissue
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Fabia Fricke

    Verified customer

    投稿 Dec 18 2017

    Thank you for contacting us. We do not know of any publications using ab67102 or ab108938. The protocol used with ab67102 is below: Blocking buffer (also Dilution buffer) Weigh non-fat milk 5 g and dissolve in 100 mL 1X PBST (0.2%) to a final mixture o...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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