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RabMAb

Anti-GPA33 抗体 [EPR4240] (ab108938)

製品の概要

  • 製品名
    Anti-GPA33 antibody [EPR4240]
    GPA33 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4240] to GPA33
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human GPA33.

  • ポジティブ・コントロール
    • HT-29, Human small intestine, and Human fetal colon lysates. Human colon tissue. Human colonic adenocarcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      May play a role in cell-cell recognition and signaling.
    • 組織特異性
      Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
    • 配列類似性
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻訳後修飾
      N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
      Palmitoylated.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A33 antibody
      • Cell surface A33 antigen antibody
      • Cell surface A33 antigen precursor antibody
      • Glycoprotein A33 (transmembrane) antibody
      • Glycoprotein A33 antibody
      • Gpa33 antibody
      • GPA33_HUMAN antibody
      • MGC129986 antibody
      • MGC129987 antibody
      • OTTHUMP00000032346 antibody
      • Transmembrane glycoprotein A33 antibody
      see all

    画像

    • All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution

      Lane 1 : HT-29 lysate
      Lane 2 : Human small intestine lysate
      Lane 3 : Human fetal colon lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
    • ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108938 showing negative staining in Breast carcinoma tissue.

    • ab108938 showing negative staining in Thyroid gland carcinoma tissue.

    • ab108938 showing positive staining in Ovarian carcinoma tissue.

    • ab108938 showing negative staining in Normal tonsil tissue.

    • ab108938 showing negative staining in Normal liver tissue.

    参考文献

    This product has been referenced in:
    • Gotanda K  et al. Circulating intestine-derived exosomal miR-328 in plasma, a possible biomarker for estimating BCRP function in the human intestines. Sci Rep 6:32299 (2016). WB, IP . Read more (PubMed: 27571936) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for contacting us. We do not know of any publications using ab67102 or ab108938. The protocol used with ab67102 is below: Blocking buffer (also Dilution buffer) Weigh non-fat milk 5 g and dissolve in 100 mL 1X PBST (0.2%) to a final mixture o...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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