Customer reviews and FAQs

製品の概要

  • 製品名Anti-GP210 antibody
    GP210 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GP210
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Xenopus laevis
    交差が予測される動物種: Mammal
  • 免疫原

    Purified bacterial N-terminal fusion protein

  • 特記事項Storage in frost-free freezers is not recommended.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium azide
    Constituent: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 210 kDa.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
  • 組織特異性Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
  • 配列類似性Belongs to the NUP210 family.
  • 翻訳後修飾N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides.
    Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
  • 細胞内局在Nucleus > nuclear pore complex. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • FLJ22389 antibody
      • GP 210 antibody
      • KIAA0906 antibody
      • Nuclear envelope pore membrane protein POM 210 antibody
      • Nuclear pore membrane glycoprotein 210 antibody
      • Nuclear pore protein gp210 antibody
      • Nucleoporin 210 antibody
      • Nucleoporin 210kDa antibody
      • Nucleoporin Nup210 antibody
      • Nucleoporin210 antibody
      • NUP 210 antibody
      • Nup210 antibody
      • PO210_HUMAN antibody
      • POM 210 antibody
      • POM210 antibody
      • Pore membrane protein of 210 kDa antibody
      see all

    Anti-GP210 antibody 画像

    • Immunocytochemistry/ Immunofluorescence - Anti-GP210 antibody (ab15600)
      Immunocytochemistry/ Immunofluorescence - Anti-GP210 antibody (ab15600)

      HeLa cells were permeabilised in PBS Triton X-100 (0.2%), fixed in 3.7% formaldehyde and blocked in 2% milk in PBS-T. The slides were incubated with 1/1000 dilution of ab15600 for 1 hour at room temperature. The slides were washed 3 times prior to incubation with the secondary antibody. ab15600 clearly stains the nuclear envelope (red). The DNA is stained with DAPI (blue).

    Anti-GP210 antibody (ab15600) 使用論文

    This product has been referenced in:
    • Fernández-Medarde A  et al. Laser microdissection and microarray analysis of the hippocampus of Ras-GRF1 knockout mice reveals gene expression changes affecting signal transduction pathways related to memory and learning. Neuroscience 146:272-85 (2007). Read more (PubMed: 17321057) »

    See 1 Publication for this product

    ab15600 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab15600 の abreview を投稿する ab15600 に関する問い合わせをする

    4 Abreviews or Q&A

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-GP210 antibody

     Below Average
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Sample African Green Monkey Cell (Kidney epithelial cell (Vero))
    Specification Kidney epithelial cell (Vero)
    Permeabilization Yes - 0.2% triton x-100
    Fixative Paraformaldehyde
    Read More
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    Username

    Eric Alonas

    Verified customer

    投稿 Nov 18 2014

    Inquiry: Hi, I am a researcher, I work with plant science. I could not find any product for this antibody related to plants. I do not know this antibody (gp210) will work with Arabidopsis or not. Because of this reason, It is possible to send me trial ...

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    Thank you very much for contacting us with your question.

    We only have full size vials of all of our products, so unfortunately we do not have any smalltrialvials to send out. Do you have the Arabidopsis amino acid sequence for GP210? I could...

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    BATCH NUMBER 101522 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal SAMPLE Xenopus oocyte PRIMARY ANTIBODY ab15600 1:1000 1:500 1:200 O/N at 4C in ringers with 2% BSA- 45 min wash DETECTION METHOD Co...

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    Thank you for the details that you have provided and for your patience. Did you also try this antibody in Western blotting? I saw a note from a colleague who spoke to you and both IHC and WB are mentioned as applications that you have tried. If yes, ca...

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    I am having trouble getting this antibody to work in xenopus in Western blot and IHC. I have significantly increased the concentration of antibody used without success.

    Thank you for contacting us. Please find links to the forms I discussed with you (one for IHC, one for Western blot). Once you have submitted these forms, we will take a look at your protocol and try to make any suggestions we think will help impro...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"