Mouse monoclonal [8D2BB12] to GOT2
Mouse, Rat, Cow, Human
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium azide
Concentration information loading...
>95% by SDS-PAGE
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration.
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
Information by UniProt
Aspartate aminotransferase 2 antibody
Immunocytochemistry/ Immunofluorescence - GOT2 antibody [8D2BB12] (ab113693)
ab113693 [8D2BB12] stained human fibroblast. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (8D2BB12, 2µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"