Mouse monoclonal [8D2BB12] to GOT2
Mouse, Rat, Cow, Human
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium azide
Concentration information loading...
>95% by SDS-PAGE
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration.
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
Information by UniProt
Aspartate aminotransferase 2 antibody
Anti-GOT2 antibody [8D2BB12] 画像
Immunocytochemistry/ Immunofluorescence - GOT2 antibody [8D2BB12] (ab113693)
ab113693 [8D2BB12] stained human fibroblast. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (8D2BB12, 2µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
Anti-GOT2 antibody [8D2BB12] (ab113693) 使用論文
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"