Anti-GOLPH3/MIDAS 抗体 (ab69171)
Key features and details
- Mouse polyclonal to GOLPH3/MIDAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GOLPH3/MIDAS antibody
GOLPH3/MIDAS 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to GOLPH3/MIDAS -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human GOLPH3/MIDAS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_071413 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69171の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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機能
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
組織特異性
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
配列類似性
Belongs to the GOLPH3/VPS74 family. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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参照データベース
- Entrez Gene: 64083 Human
- Omim: 612207 Human
- SwissProt: Q9H4A6 Human
- Unigene: 408909 Human
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別名
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
画像
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All lanes : Anti-GOLPH3/MIDAS antibody (ab69171) at 1/500 dilution
Lane 1 : GOLPH3/MIDAS transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab69171 は 4 報の論文で使用されています。
- Wang K et al. GOLPH3 Promotes Cancer Growth by Interacting With STIP1 and Regulating Telomerase Activity in Pancreatic Ductal Adenocarcinoma. Front Oncol 10:575358 (2020). PubMed: 33134174
- Chen S et al. Widespread and Functional RNA Circularization in Localized Prostate Cancer. Cell 176:831-843.e22 (2019). PubMed: 30735634
- Li H et al. GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer. J Transl Med 10:168 (2012). WB . PubMed: 22905766
- Wang JH et al. High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis. PLoS One 7:e45622 (2012). WB, IHC . PubMed: 23056210