製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Golgin 97
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids 18-67 (RPGGATRIPR SVSKESVASM GADSGDDFAS DGSSSREDLS SQLLRRNEQI) of Human Golgin 97 (NP_002068).

  • ポジティブ・コントロール
    • HCT15 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Constituents: 98% PBS, 2% Sucrose
  • Concentration information loading...
  • 精製度
    >95% by SDS-PAGE
  • 特記事項(精製)
    Affinity purifications with biochemical fractionation.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 88 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-Golgin 97 antibody (ab112084) at 1 µg/ml + HCT15 cell lysate at 10 µg

    Predicted band size : 88 kDa

プロトコール

参考文献

ab112084 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole heart lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-15%)
Loading amount
30 µg
Specification
Whole heart lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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