製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to GOLGA5
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human GOLGA5, amino acids 1-731 (NP_005104.2)

  • ポジティブ・コントロール
    • Human liver tisssue lysate, HepG2 cell lysate, GOLGA5 transfected 293T cell lysate and HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
  • 組織特異性
    Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.
  • 関連疾患
    Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving GOLGA5 is found in thyroid papillary carcinomas. Translocation t(10;14)(q11;q32) with RET. The translocation generates the RET/GOLGA5 (PTC5) oncogene which was found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl.
  • 翻訳後修飾
    Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase.
  • 細胞内局在
    Golgi apparatus membrane. Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation inducing gene 31 antibody
    • Cell proliferation-inducing gene 31 protein antibody
    • GOGA5_HUMAN antibody
    • GOLGA 5 antibody
    • GOLGA5 antibody
    • Golgi autoantigen golgin subfamily a 5 antibody
    • Golgi integral membrane protein 5 antibody
    • Golgin 84 antibody
    • Golgin A5 antibody
    • Golgin subfamily A member 5 antibody
    • Golgin-84 antibody
    • Golgin84 antibody
    • GOLIM 5 antibody
    • GOLIM5 antibody
    • Protein Ret-II antibody
    • PTC5 antibody
    • Ret fused gene 5 antibody
    • RET fused gene 5 protein antibody
    • Ret II antibody
    • Ret II protein antibody
    • RET-fused gene 5 protein antibody
    • RFG 5 antibody
    • RFG5 antibody
    see all

画像

  • Anti-GOLGA5 antibody (ab89136) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • Anti-GOLGA5 antibody (ab89136) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • All lanes : Anti-GOLGA5 antibody (ab89136) at 1 µg/ml

    Lane 1 : GOLGA5 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • ab89136 at 10µg/ml staining GOLGA5 in HeLa cells by Immunofluorescence.

参考文献

ab89136 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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