製品の概要

  • 製品名Anti-GODZ antibody
    GODZ 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GODZ
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse GODZ.

    (Peptide available as ab31882.)

  • ポジティブ・コントロール
    • Mouse Cerebellum Tissue Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19001095
IHC-P 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

ターゲット情報

  • 機能Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi.
  • 配列類似性Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • ドメインThe DHHC domain is required for palmitoyltransferase activity.
  • 翻訳後修飾Autopalmitoylated.
  • 細胞内局在Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DHHC-3 antibody
    • GABA-A receptor-associated membrane protein 1 antibody
    • Golgi-specific DHHC zinc finger protein antibody
    • Gramp1 antibody
    • Palmitoyltransferase ZDHHC3 antibody
    • Protein DHHC1 antibody
    • ZDHC3 antibody
    • ZDHC3_HUMAN antibody
    • ZDHHC3 antibody
    • Zinc finger DHHC domain-containing protein 3 antibody
    • Zinc finger protein 373 antibody
    • ZNF373 antibody
    see all

Anti-GODZ antibody 画像

  • (Image courtesy of Human Protein Atlas)
    ab31837 detecting GODZ protein in the purkinje cells of normal human cerebellum. Paraffin embedded human cerebral cortex tissue was incubated with ab31837 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

  • Anti-GODZ antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-GODZ antibody (ab31837) 使用論文

This product has been referenced in:
  • Levy AD  et al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases. Mol Biol Cell 22:1930-42 (2011). Read more (PubMed: 21471001) »
  • Shmueli A  et al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF ; Human . Read more (PubMed: 19927128) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

Here are the links to the Human Protein Atlas as well as to the mouse protein entry in UniProt database:

http://www.proteinatlas.org/ENSG00000163812/cell
http://www.proteinatlas.org/ENSG00000163812
Read More

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"