By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. Cross reactivity with
IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less
than 1% cross reactivity to human and mouse IgM was detected. This antibody may cross react with IgG from other species.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/500.
1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
1/50 - 1/200.
DyLight®-Goat polyclonal Secondary Antibody to Rat IgM - mu chain (DyLight® 650), pre-adsorbed(ab98371)
Emission spectra of DyLight® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
This product has been referenced in:
Moore JB et al. Epigenetic reprogramming and re-differentiation of a Ewing sarcoma cell line. Front Cell Dev Biol3:15 (2015).
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