Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed (ab98368)

製品の概要

  • 製品名Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed
    IgM 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Rat IgM - mu chain (DyLight® 488), pre-adsorbed
  • ターゲット生物種Rat
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgM was detected. This antibody may cross react with IgG from other species.
  • アプリケーション適用あり: IHC-P, ICC/IF, Flow Cytmore details
  • 吸着処理血清
    Human, Mouse more details
  • 標識DyLight® 488. Ex: 493nm, Em: 518nm

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab98368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed 画像

  • Overlay histogram showing THP1 cells stained with ab16081 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16081, 1µg/1x10^6 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgM (mu chain) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Rat IgM [RTK2118] (ab35768, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed (ab98368) 使用論文

ab98368 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab98368.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"