Goat anti-Rat IgG H&L (PE) secondary 抗体 (ab7010)

製品の概要

  • 製品名Goat anti-Rat IgG H&L (PE) secondary antibody
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rat
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 吸着処理血清
    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • 免疫原

    Full length native Rat IgG (purified).

  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 特記事項(標識)R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.

Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.

Goat anti-Rat IgG H&L (PE) secondary antibody (ab7010) 使用論文

This product has been referenced in:
  • Zhang ZH  et al. An integrated lipidomics and metabolomics reveal nephroprotective effect and biochemical mechanism of Rheum officinale in chronic renal failure. Sci Rep 6:22151 (2016). IHC-P . Read more (PubMed: 26903149) »
  • Kim SM  et al. Acquired resistance to cetuximab is mediated by increased PTEN instability and leads cross-resistance to gefitinib in HCC827 NSCLC cells. Cancer Lett 296:150-9 (2010). Flow Cyt . Read more (PubMed: 20444542) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and for bringing this matter to my attention. A mistake appears to have occurred on our product data sheet and the value should read ug and not mg. I have updated the product data sheet to reflect this change. I apologise for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"