Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)
Key features and details
- Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
アプリケーション
適用あり: Flow Cyt, ICC/IF, IHC-Pmore details -
吸着処理血清
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
標識
DyLight® 488. Ex: 493nm, Em: 518nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab98420の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
1/50 - 1/200.
|
|
ICC/IF |
1/50 - 1/500.
|
|
IHC-P |
1/50 - 1/500.
|
特記事項 |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
画像
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima. -
ICC/IF image of ab6161 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rat- H&L, pre-adsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab98420 は 5 報の論文で使用されています。
- Zhang J et al. Giant proteins in a giant cell: Molecular basis of ultrafast Ca2+-dependent cell contraction. Sci Adv 9:eadd6550 (2023). PubMed: 36812318
- Yoo B et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). PubMed: 30886310
- Yoo B et al. MicroRNA-710 regulates multiple pathways of carcinogenesis in murine metastatic breast cancer. PLoS One 14:e0226356 (2019). PubMed: 31834924
- Shrestha RL et al. TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes. Open Biol 4:130108 (2014). ICC/IF . PubMed: 24898139
- Liegel RP et al. Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans. Am J Hum Genet 93:1001-14 (2013). PubMed: 24239381