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Our Abpromise guarantee covers the use of ab96974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.|
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
Anti-dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974) was used as a secondary antibody at a concentration of 1/500.
Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells permeabilized with 0.2% Triton X-100 using ab96974 (red) at 1/500 in PBS + 2% BSA for 1 hour.
ab96974 has not yet been referenced specifically in any publications.