Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (ab96974)
Key features and details
- Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.
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アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cytmore details -
吸着処理血清
Human, Mouse more details -
標識
DyLight® 650. Ex: 654nm, Em: 673nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
DyLight® 650 replaces DyLight® 649. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96974の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/500.
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ICC/IF | (1) |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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特記事項 |
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IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
画像
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Anti-dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974) was used as a secondary antibody at a concentration of 1/500.
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Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells permeabilized with 0.2% Triton X-100 using ab96974 (red) at 1/500 in PBS + 2% BSA for 1 hour.
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab96974 は論文での使用が確認できていません。