Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (ab96974)

製品の概要

  • 製品名Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed
  • ターゲット生物種Rat
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
  • アプリケーション適用あり: IHC-P, ICC/IF, Flow Cytmore details
  • 吸着処理血清
    Human, Mouse more details
  • 標識DyLight® 650. Ex: 654nm, Em: 673nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項DyLight® 650 replaces DyLight® 649.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed 画像

  • Anti-dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 650), pre-adsorbed (ab96974) was used as a secondary antibody at a concentration of 1/500.

  • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells permeabilized with 0.2% Triton X-100 using ab96974 (red) at 1/500 in PBS + 2% BSA for 1 hour.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Rat IgG Fc (DyLight® 650) preadsorbed (ab96974) 使用論文

ab96974 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
red channel is anti-Rat Dy650, permeablize using 0.2%TritonX-100. work at dilution of 1:500, incubate in 2%BSA/PBS at RT for 1h
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Ms. YA ZHOU

Verified customer

投稿 Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"