Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)

製品の概要

  • 製品名
    Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Rat
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, Flow Cytmore details
  • 吸着処理血清

    Human, Mouse more details
  • 標識
    DyLight® 550. Ex: 562nm, Em: 576nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 特記事項
    DyLight® 550 replaces DyLight® 549.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed 画像

  • Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972) was used as a secondary antibody at a concentration of 1/500.
  • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972) 使用論文

ab96972 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
yellow channel is anti-Rat Dy550, label a cell surface protein, no permeablization. work at dilution of 1:500, incubate in 2%BSA/PBS at RT for 1h
Username

Ms. Ya Zhou

Verified customer

投稿 Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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