By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/500.
1/50 - 1/500.
1/50 - 1/200.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.
Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972)
was used as a secondary antibody at a concentration of 1/500.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)This image is courtesy of an Abreview submitted by Ya Zhou
Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.
DyLight® - Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972)
Emission spectra of DyLight® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
has not yet been referenced specifically in any publications.