Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

製品の概要

  • 製品名Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Rabbit IgG (PE), pre-adsorbed
  • ターゲット生物種Rabbit
  • アプリケーション適用あり: Flow Cyt, ELISAmore details
  • 吸着処理血清
    Cow, Mouse more details
  • 免疫原

    Rabbit IgG

  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1.64% Sodium phosphate, 1.71% Sucrose, 0.87% Sodium chloride, 0.1% BSA
  • Concentration information loading...
  • 精製度Size exclusion
  • 特記事項(精製)Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • 特記事項(標識)Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/500 - 1/1000.
ELISA Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed 画像

  • Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.

  • Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465

Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

This product has not been tested in ICC and so while we cannot guarantee that it will work in that application, we see no reason to believe that it wouldn't. We would of course recommend thoroughly blocking yo...

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Application Flow Cytometry
This secondary antibody was used to detect Slc4a1 on mouse erythrocytes as control as well as differentiated mouse embryonic stem cells. It can be seen in the picture attached that the labelling detected with this secondary antibody alone is practically identical to that obtained with un-labelled cells, meaning that the background is very low, which increases the confidence in the specificity of the primary antibody used.
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Alena Pance

Verified customer

投稿 Sep 08 2014

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is ...

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Thank you for your reply.
Below are the 2 products that I would recommend for you:
Isotype control - http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html
Secondary - http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H...

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Thank you for contacting Abcam.
I would be happy to help you select the best products for your experiments. To be able to assist you further, would you please let me know which applications you wish to use these products in. That way I can recommen...

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Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - ra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"