Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

Abreviews (2)Q&A (4)Specific References (7)
Customer reviews and FAQs

製品の概要

  • 製品名
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項（精製）
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • 特記事項（標識）
    Horseradish Peroxidase (HRP)
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/20000 - 1/80000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)This image is courtesy of an anonymous Abreview.

    ab9986 staining osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA and 5% normal goat serum in PBS for 30 minutes at 22°C; Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab7090) (1/300) was used as the secondary antibody.

    See Abreview

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) 使用論文

This product has been referenced in:
  • Bi Y  et al. MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma. Int J Mol Med 39:900-906 (2017). WB ; Human . Read more (PubMed: 28259961) »
  • Esbona K  et al. COX-2 modulates mammary tumor progression in response to collagen density. Breast Cancer Res 18:35 (2016). IHC-P . Read more (PubMed: 27000374) »

See all 7 Publications for this product

ab7090 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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ab7090 の abreview を投稿する ab7090 に関する問い合わせをする

6 Abreviews or Q&A

10
Votes: Highest First

Good abtibody

 Excellent
Abreviews
Application
Western blot
I use this antibody mostly in WB. I can get good signals (1/10000 dilution).

Upload image condition:
1st antibody ab108296 1/1000 dilution(5% milk)
2nd antibody This antibody 1/10000 dilution(5% milk)
abreview image
Username

Abcam user community

Verified customer

投稿 Sep 14 2017

Immunochemistry with ab7090

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This is an example of immunochemistry anti-Inos (ab15323) with Goat anti Rabbit HRP (on human artery)
-Deparaffinize
-heat in citrate buffer 20min at 96°C
-H2O2 for 10min at room temperature
-Block with PBS BSA 1% goat serum 5% at least 1h at room temperature
-incubate anti INOS at 1/100 in PBS BSA Overnight at 4°C
-incubate with goat anti rabbit HRP (1/300) for 45min at 37°C
-incubate slides in DAB or other peroxidase substrate
-counterstain with hematoxylin, dehydrate and mount
abreview image
Username

Abcam user community

Verified customer

投稿 Jan 29 2014

Dear abcam technical support team: This customer already purchases ab80436 (EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit) and she wants to buy the “Goat anti-rabbit HRP Conjugate” alone, could you please offer any suitable selection to he...

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Thank you for contacting us.

The Goat anti-rabbit HPR Conjugated used in AB80436 has been developed specifically for use in this kit and is currently not available for separate purchase. We do offer a number of Goat anti-rabbit secondaries...

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I have addressed your questions below:

1. The details you provide indicate several secondaries are giving a band at 75 kDa in the no primary control tests. Please confirm further details of any other secondary antibodies you are having this ...

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Thank you for your reply.

Reviewing the details, if you obtaining a band at 75 kDa in the no primary control with many secondary antibodies with the rat kidney lysate, but not the other samples, then I would recommend there is something in t...

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Hello technical support,

Recently we purchased two secondary antibodies from abcam to use for western blotting and we are getting a dark band at 75 KD with secondary antibody alone. Can you please advise me on what I should do next.

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these two secondaryantibody.

The details you have kindly provided will enable us to investigate this case for you and...

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Mr I have some questions about antibodie IgG-ab7090. This antibodie no cross-reactive with mouse monoclonal antibodies (primary antibodies)? How is the difference with secondary rabbit antibodies no pre-adsorbed? I need in my group a secondary r...

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This antibody has been pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This minimises the cross reactivity of this antibody with these proteins. However, minimal cross reactivity will still be ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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