製品の概要

  • 製品名Goat Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 特記事項(標識)Molar enzyme/ antibody protein ratio is 4:1
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項Part of the AbExcel range.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Rabbit IgG H&L (HRP) 画像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) 使用論文

This product has been referenced in:
  • Recha-Sancho L & Semino CE Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering. PLoS One 11:e0157603 (2016). Read more (PubMed: 27315119) »
  • Thirunavukkarasu S  et al. Cytochrome P450 1B1 Contributes to the Development of Angiotensin II-Induced Aortic Aneurysm in Male Apoe(-/-) Mice. Am J Pathol N/A:N/A (2016). Read more (PubMed: 27301358) »

See all 32 Publications for this product

Product Wall

Gracias por tu respuesta. Me alegra oír que este secundario os ha dado buenos resultados.

Ya he realizado por tanto el nuevo pedido completamente gratuito del mismo anticuerpo: ab97051.

Recibiréis una confirmació...

Read More

Thank you for passing some information to me.
I understand that ab3583 has been used previously at 1:4000.
Have you had a chance to test this antibody at dilutions of 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC?
Wou...

Read More
Application Western blot
Secondary antibody (ab97051) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for JNK1/2 antibody from non abcam brand. Though the secondary antibody could detect a band at expected 42 kD,
There was a non specific band (around 40-55 kD) was shown by the secondary antibody when used without primary antibody incubation also.
gel: 12%, 200 V for 45 min.
transfer 17 V for 35 min. using Biorad SD.
Blocking 5% BSA.
Username

Vicky

Verified customer

投稿 Oct 10 2016

Application Western blot
this antibody works stably at a dilution of 1:4,000 for western blot assay.
Username

Abcam user community

Verified customer

投稿 Feb 08 2016

Abreviews
Application Western blot
always works for WB at a dilution of 1:5,000.
Username

Abcam user community

Verified customer

投稿 Nov 02 2015

Abreviews
Application Western blot
We stock and use this for many of our westerns, gets the job done!
("B" and "G" are brain and gonad samples.)
Username

Dr. Sebastian Alvarado

Verified customer

投稿 Aug 21 2015

Abreviews
Application Western blot
1. The concentration of Goat Anti-Rabbit IgG H&L (HRP) used for this experiment is 1:5000 and incubated for 2 hours.
2. Proteins were blocked with 5% BSA in TBS with 0.1% TritonX-100.
Username

Mr. Lenin Veeraval

Verified customer

投稿 Jun 18 2015

Application Western blot
Abcam's anti-rabbit HRP is very reliable and economical to use. We ended up using 10x less than our old antibody from a different company. Since it is so robust, we started calling it the "epic rabbit antibody" to distinguish it from the old one we have in the lab. The lower band in the gel corresponds to beta-tubulin (ab6046) followed by anti-rabbit HRP (ab97051) while the upper band is a different 70kDa protein followed by anti-mouse HRP (ab97023).
Username

Abcam user community

Verified customer

投稿 Aug 21 2014

Application Western blot
Secondary worked very well. Used at 1:5000.
Username

Abcam user community

Verified customer

投稿 Aug 15 2014

This product has been tested using ECL and will perform very well using this detection system.

1-10 of 35 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"