Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)

製品の概要

  • 製品名
    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed
  • ターゲット生物種
    Rabbit
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, Flow Cytmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • 標識
    DyLight® 650. Ex: 654nm, Em: 673nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 特記事項
    DyLight® 650 replaces DyLight® 649.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed 画像

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902) 使用論文

This product has been referenced in:
  • Romanelli D  et al. Roles and regulation of autophagy and apoptosis in the remodelling of the lepidopteran midgut epithelium during metamorphosis. Sci Rep 6:32939 (2016). IF . Read more (PubMed: 27609527) »
  • Shenkman M  et al. A shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins. J Biol Chem 288:2167-78 (2013). ICC/IF . Read more (PubMed: 23233672) »

See all 3 Publications for this product

Product Wall

As discussed over the phone I have looked at the protocols we have for double staining to see if I can suggest anything further to our conversation. As discussed the protocol that we have on our website is relatively simple, with sequential incubation ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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