Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886)

製品の概要

  • 製品名Goat Anti-Rabbit IgG H&L (DyLight® 650)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • アプリケーション適用あり: IHC-P, ICC/IF, Flow Cytmore details
  • 標識DyLight® 650. Ex: 654nm, Em: 673nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項DyLight® 650 replaces DyLight® 649.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rabbit IgG H&L (DyLight® 650) 画像

  • ab10559 staining CD45 in pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A DyLight® 650-conjugated Goat anti-rabbit IgG H&L (ab96886) (1/500) was used as the secondary antibody.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886) 使用論文

ab96886 has not yet been referenced specifically in any publications.

Product Wall

excellent

Excellent
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Good for internalization experiment, used at 1:500, red channel is surface GFP labelled in red, green channel is internalized GFP labelled with AB96886, it's invisible by eyes, but perfect for confocal imaging
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Ms. YA ZHOU

Verified customer

投稿 Oct 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"