Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886)
Key features and details
- Goat Anti-Rabbit IgG H&L (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (DyLight® 650)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cytmore details -
標識
DyLight® 650. Ex: 654nm, Em: 673nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
DyLight® 650 replaces DyLight® 649. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96886の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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ICC/IF | (1) |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
|
特記事項 |
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
画像
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ab10559 staining CD45 in pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A DyLight® 650-conjugated Goat anti-rabbit IgG H&L (ab96886) (1/500) was used as the secondary antibody.
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (9)
ab96886 は 9 報の論文で使用されています。
- Minderman M et al. The oncogenic human B-cell lymphoma MYD88 L265P mutation genocopies activation by phosphorylation at the Toll/interleukin-1 receptor (TIR) domain. Blood Cancer J 13:125 (2023). PubMed: 37591861
- Riemma MA et al. Sphingosine-1-phosphate/TGF-β axis drives epithelial mesenchymal transition in asthma-like disease. Br J Pharmacol 179:1753-1768 (2022). PubMed: 34825370
- Lee CW et al. Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells. BMC Mol Cell Biol 23:26 (2022). PubMed: 35794526
- Reddy HP et al. Encephalopathy-causing mutations in Gß1 (GNB1) alter regulation of neuronal GIRK channels. iScience 24:103018 (2021). PubMed: 34522861
- Tabak G et al. Mutual action by G? and Gß for optimal activation of GIRK channels in a channel subunit-specific manner. Sci Rep 9:508 (2019). PubMed: 30679535
- Jacenik D et al. G protein-coupled estrogen receptor mediates anti-inflammatory action in Crohn's disease. Sci Rep 9:6749 (2019). PubMed: 31043642
- Telegina DV et al. Mechanisms of Neuronal Death in the Cerebral Cortex during Aging and Development of Alzheimer's Disease-Like Pathology in Rats. Int J Mol Sci 20:N/A (2019). PubMed: 31717998
- Stefanova NA et al. An antioxidant specifically targeting mitochondria delays progression of Alzheimer's disease-like pathology. Aging (Albany NY) 8:2713-2733 (2016). PubMed: 27750209
- Stefanova NA et al. Melatonin attenuates impairments of structural hippocampal neuroplasticity in OXYS rats during active progression of Alzheimer's disease-like pathology. J Pineal Res 59:163-77 (2015). PubMed: 25988948