アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Other Immunogen Type corresponding to Rabbit IgG.
Our Abpromise guarantee covers the use of ab97075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.|
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/100 - 1/1000.|
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3®) (ab97075), 1:100
Nuclei were counterstained with SYTO 16
Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse endoderm cell
Fix in 3% PFA in PBS for 30 min at RT
Primary antibody: Rabbit anti-talin (ab71333), 1:100
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100
ab97075 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"