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Other Immunogen Type corresponding to Rabbit IgG.
Our Abpromise guarantee covers the use of ab97075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500.|
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/100 - 1/1000.|
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3®) (ab97075), 1:100
Nuclei were counterstained with SYTO 16
Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse endoderm cell
Fix in 3% PFA in PBS for 30 min at RT
Primary antibody: Rabbit anti-talin (ab71333), 1:100
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100