Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)


  • 製品名Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed
  • ターゲット生物種Rabbit
  • アプリケーション適用あり: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 標識Cy3 ®. Ex: 552nm, Em: 565nm


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  • 特記事項(標識)Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

  • 研究分野


Our Abpromise guarantee covers the use of ab6939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-P 1/100. PubMed: 17045908
ICC Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed 画像

  • Ab6939 was used at dilution 1/500 with the primary antibody ab10812 in ICC/IF. See the review on ab10812.

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939) 使用論文

This product has been referenced in:
  • Islam R  et al. Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes. PLoS One 10:e0128306 (2015). ICC ; Human . Read more (PubMed: 26052937) »
  • Buchholz M  et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS One 10:e0122946 (2015). ICC . Read more (PubMed: 25849100) »

See all 20 Publications for this product

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It is clear from the images provided that the customer has observed some background with the secondary antibody. In order to reduce this, I would advise reducing the concentration which is currently being use...

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