Goat Anti-Rabbit IgG H&L (AMCA) (ab123435)

製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.20
    Preservative: 0.013% Sodium azide
    Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(標識)Blue fluorescence. Excitation/Emission wavelength= 344 nm/ 446 nm
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 - 30 µg/ml.
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (AMCA) 画像

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-EndoA alpha 1, 1:100
    Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained with SYTO 16

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained stained with DRAQ7TM (ab109202)

Goat Anti-Rabbit IgG H&L (AMCA) (ab123435) 使用論文

ab123435 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is ...

Read More

Thank you for contacting us.

Unfortunately we do not havesecondary antibodies conjugated to Alexa 405 or Pacific Blue.
However, we have the following secondary antibodies, conjugated to AMCA, a blue fluorescence, Excitation/Emission wavele...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"