Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)

製品の概要

  • 製品名Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Rabbit
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 標識Alkaline Phosphatase

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • 特記事項(標識)Molar enzyme: antibody protein ratio is 1:1
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項Part of the AbExcel range.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. Primary
WB 1/5000 - 1/50000. Chemiluminescent detection

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) 画像

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate 
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/5000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/20000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048) 使用論文

This product has been referenced in:
  • Jeong KI  et al. CX3CR1 Is Expressed in Differentiated Human Ciliated Airway Cells and Co-Localizes with Respiratory Syncytial Virus on Cilia in a G Protein-Dependent Manner. PLoS One 10:e0130517 (2015). WB ; Human . Read more (PubMed: 26107373) »
  • Mehrbod P  et al. Simvastatin modulates cellular components in influenza A virus-infected cells. Int J Mol Med 34:61-73 (2014). WB . Read more (PubMed: 24788303) »

See all 4 Publications for this product

Product Wall

MVH

Excellent
Abreviews
Application Western blot
30ug of oocyte lysate with RIPA
primary1:1000
12hrs 4deg
Secondary 1:2000
1hr room temp
detection with alkaline phosphatase substrate
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Abcam user community

Verified customer

投稿 Jul 06 2016

Thank you for contacting us and your interest in our products.

The Anti-Osteopontin antibody (ab8448) and the Goat anti-Rabbit IgG H&L (AP) secondary antibody (ab97048) should be suitable for the experiment you are planning. They have bot...

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Thank you for contacting us.


As the secondary Goat anti-Rabbitantibody was raised against the whole heavy and light chains of theIgG molecule, and as a Fab2 fragment contains both as well, I don't see way the antibody shouldn't react. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"