Goat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed (ab27237)

製品の概要

  • 製品名
    Goat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Rabbit
  • アプリケーション
    適用あり: Electron Microscopymore details
  • 吸着処理血清

    Human more details
  • 免疫原

    Full length native Rabbit IgG

  • 標識
    Gold 20nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 20% Glycerol, 1% BSA, Tris buffered saline. pH 8.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Electron Microscopy
  • 追加情報
    Electron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 画像

    • ab27237 used in Electron Microscopy.
      Subcellular localization of RIG-I in human neutrophils.

      C: Isolated peripheral blood neutrophils were fixed, washed with PBS, permeabilized, and blocked (10% normal goat serum, 10 mm glycine, and 0.2% Tween 20 in diluent containing 0.5% bovine serum albumin and 0.5% Triton X-100 in PBS). For visualization of RIG-I, the grids were incubated with anti-RIG-I antibody and a gold-conjugated secondary antibody (gold sphere diameter, 5 nm; ab27237, Abcam). Finally, the grids were stained with 1% uranyl acetate. Negative controls were obtained by omitting the primary antibody.

      Arrows indicate RIG-I staining. The red arrows mark RIG-I associated with a vesicle-like structure.

    参考文献

    This product has been referenced in:
    • Marcos V  et al. Free DNA in cystic fibrosis airway fluids correlates with airflow obstruction. Mediators Inflamm 2015:408935 (2015). Read more (PubMed: 25918476) »
    • Riopel M  et al. Ultrastructural and immunohistochemical analysis of the 8-20 week human fetal pancreas. Islets 6:e982949 (2014). Read more (PubMed: 25425025) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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