製品の概要

  • 製品名Goat Anti-Rabbit IgG Fc (HRP)
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Rabbit IgG - Fc (HRP)
  • ターゲット生物種Rabbit
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG.
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 標識HRP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 特記事項(標識)Molar enzyme/ antibody protein ratio is 4:1
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary ELISA)
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

Goat Anti-Rabbit IgG Fc (HRP) 画像

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab97200 was used at dilution 1/5000 with the primary antibody ab90045 in WB. See the review on ab90045.

Goat Anti-Rabbit IgG Fc (HRP) (ab97200) 使用論文

This product has been referenced in:
  • Chen B  et al. Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13:2527-35 (2016). WB . Read more (PubMed: 26848028) »
  • Ning P  et al. Role of protein S in castration-resistant prostate cancer-like cells. Endocr Relat Cancer 23:595-607 (2016). Read more (PubMed: 27342144) »

See all 13 Publications for this product

Product Wall


I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:
http://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hr...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab7778 bisher noch nicht inSchwein getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot...

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Thank youf or your inquiry.

We have many goat anti rabbit IgG HRP conjugated antibodies:

Please use the links below to review the datasheets of three of them:

ab97051

http://www.abcam.com/index.html?datasheet=970...

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Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not...

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We have been informed by the source of the secondary antibodies ab97195 and ab97200 that : By immunoelectrophoresis and ELISA, ab97195 (anti-Rabbit IgM - HRP) reacts specifically with rabbit IgM. This antibody may cross react with IgM from other spec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"