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Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)

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Overview

Product name

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP)
IgG 抗体 (1691件) 一覧

Target species

Rabbit

Tested applications

IHC-P, WB, ELISA, Dot Blot, ICC/IF, IHC-Fr, ICCmore details

Immunogen

Rabbit IgG whole molecule

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8.0

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation notes

Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Clonality

Polyclonal

Research areas

Secondary antibodies >> anti-Rabbit >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

  • Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722) image (enlarge)

  • ELISA - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)ELISA - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) images:

  Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)

Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)

Ab6722 was used at dilution 1/10000 with the primary antibody ab16731 in WB. See the review on ab16731.

  ELISA - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)

ELISA - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)

Ab6722 was used with the primary antibody ab2594 in ELISA. See Abreview on ab2594.

Protocols

  • ELISA procedure for ab6722

    • References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab6722)

    This product has been referenced in:

    • Kalamajski S & Oldberg A Homologous sequence in lumican and fibromodulin leucine-rich repeat 5-7 competes for collagen binding. J Biol Chem 284:534-9 (2009). Indirect ELISA.Read more (PubMed: 19008226) »
    • Häger Met al. Cib2 binds integrin alpha7Bbeta1D and is reduced in laminin alpha2 chain-deficient muscular dystrophy. J Biol Chem 283:24760-9 (2008).Read more (PubMed: 18611855) »

    See all 6 publications for this product

    Publishing research using ab6722? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"