Goat Anti-Mouse IgM mu chain (Agarose) (ab65867)

製品の概要

  • 製品名Goat Anti-Mouse IgM mu chain (Agarose)
    IgM 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • 特異性Specificity for the mu-chain of mouse IgM is determined by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses) and IgM myeloma proteins. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to agarose bead coupling. Electrophoresis of the product followed by diffusion against anti-goat IgG and anti-goat whole serum results in single arcs of precipitation in the gamma region.
  • 免疫原

    Mouse IgM

  • 標識Agarose

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituent: 2.9% Sodium chloride
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(標識)The IgG fraction of goat anti-mouse IgM (mu-chain specific) antiserum is covalently attached to cyanogen bromide activated cross-linked beaded agarose. Five to ten milligrams of IgG fraction is bound per milliliter of resin. After equilibration, a minimum of 0.4 mg of mouse IgM can be bound and eluted per milliliter of packed resin.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項Procedure
    A two milliliter column of antibody-agarose is prepared using four milliliters of the antibody-agarose suspension. The column is equilibrated in 0.01 M sodium phosphate buffer, pH 7.2, containing 0.5 M NaCl (PB). The antigen solution to be bound is applied slowly and followed by a PB wash. Unbound effluent fractions are collected and assayed for protein content (Lowry). The column is then stripped by washing with 0.1 M glycine, 0.15 M NaCl, pH 2.4, or 0.5 M acetic acid, 0.15 M NaCl, pH 2.4. Fractions containing protein are collected, brought to neutral pH and assayed for protein content (Lowry).

    Regeneration
    Goat Anti-Mouse IgM-Agarose may be regenerated and used for future adsorptions. Strip the agarose with ten column volumes of 0.1 M glycine, 0.15 M sodium chloride, pH 2.4, or 0.5 M acetic acid, 0.15 M sodium chloride, pH 2.4, then wash with 0.01 M sodium phosphate buffer, pH 7.2, containing 0.5 M sodium chloride (PB).
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
AP Use at an assay dependent dilution.

Goat Anti-Mouse IgM mu chain (Agarose) (ab65867) 使用論文

ab65867 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam technical services regarding ab65867 - Anti-Mouse IgM.   I can confirm that this product has been tested for affinity purification only and we haven't tested it for immunoprecipitation specifically. As the principle i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"