Goat Anti-Mouse IgG2b heavy chain (Biotin) (ab97248)
Key features and details
- Goat Anti-Mouse IgG2b heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgG2b heavy chain (Biotin)
IgG2b 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with other immunoglobulins is less than 2%. -
アプリケーション
適用あり: IHC-P, ICC/IF, ELISA, WBmore details -
標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97248の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/250 - 1/2500.
|
|
ICC/IF |
1/100 - 1/500.
|
|
ELISA |
1/10000 - 1/200000. ELISA-Primary
|
|
WB |
1/10000 - 1/200000. WB-Chemiluminescent
|
特記事項 |
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IHC-P
1/250 - 1/2500. |
ICC/IF
1/100 - 1/500. |
ELISA
1/10000 - 1/200000. ELISA-Primary |
WB
1/10000 - 1/200000. WB-Chemiluminescent |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab97248 は 2 報の論文で使用されています。
- Schetters STT et al. Immunological dynamics after subcutaneous immunization with a squalene-based oil-in-water adjuvant. FASEB J 34:12406-12418 (2020). PubMed: 33411367
- Schetters STT et al. Mouse DC-SIGN/CD209a as Target for Antigen Delivery and Adaptive Immunity. Front Immunol 9:990 (2018). PubMed: 29867967