Goat Anti-Mouse IgG1 heavy chain (HRP) preadsorbed (ab98693)

Abreviews (1)Q&A (17)Specific References (5)

製品の概要

  • 製品名
    Goat Anti-Mouse IgG1 heavy chain (HRP) preadsorbed
    IgG1 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP), pre-adsorbed
  • 由来種
    Goat
  • ターゲット生物種
    Mouse
  • アプリケーション
    適用あり: ICC, IHC-P, ELISA, WBmore details
  • 吸着処理血清

    Human, Rat more details
  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Similar dilution to primary antibody.
WB 1/2000 - 1/20000. Colorimetric 1/5000 - 1/25000 Chemiluminescent

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 heavy chain (HRP) preadsorbed (ab98693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 heavy chain (HRP) preadsorbed (ab98693)

    IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab98693, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

参考文献

This product has been referenced in:
  • Gulati S  et al. Attenuation of opioid tolerance by ETB receptor agonist, IRL-1620, is independent of an accompanied decrease in nerve growth factor in mice. Heliyon 3:e00317 (2017). WB ; Mouse . Read more (PubMed: 28626808) »
  • Liu SA  et al. Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice. J Virol 91:N/A (2017). ELISA . Read more (PubMed: 28404844) »

See all 5 Publications for this product

ab98693 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

18 reviews or Q&A

10
Votes: Highest First

Western blot using the antibody with cell lysate prepared from S. cerevisiae.

 Excellent
Abreviews
Application
Western blot
Myc tagged protein 65kDa probed with Monoclonal Myc antibody and later with Mouse secondary Heavy chain specific HRP. Lane 5 is negative control with no Myc tagged protein. The antibody is excellent and recognized the specific band. I highly recommend this antibody.
abreview image
Username

Abcam user community

Verified customer

投稿 Feb 28 2014

To whom it may concern

We are evaluating gamma delta and CD4+Tcells in chicken’s section by IHC technique and we purchase your primary antibodies (ab25347 and ab25345) and secondary HRP antibody (ab98693).

After looking your web...

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Thank you for contacting us.

The primary antibodies ab25345 and ab25347 you purchased are purified antibodies. We recommend to start with a concentration of 5 ug/ml for ICC and IHC. According to the results the concentration might need some op...

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ab70: will test in ELISA
send DS link for ab98693

Thank you for your phone call.

The link to the datasheet of the secondary antibody is :

http://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-hrp-pre-adsorbed-ab98693.html


Here is the discount...

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Our customer related that the liquid was all over the pouch.
Abcam Order # 1119930 -- July 09th 2012

Thanks and best regards

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Hello
Our customer received the product ab98693 (PO 123642) damaged.
Is it possible to send another?
Attached the purchase document (#2)
Thanks and best regards

Thank you for contacting us.

Could you provide more details about the problem?

- was the vial broken
- was the liquid all over the pouch
- When the product was ordered. Could you provide Abcam purchase order number? The purcha...

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thanks for the reply, could you please confirm the products ab98963,ab97245 andab97250 can be used asELSIAsecondary antibodies.

Thank you for your email.

These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

Just let me know if you have further questions.

Thanks for your kindly reply
about my first question, i want to know after incubation of secondary antibody (ab98693) is there any necessity for substrate? do we need anythings else other than DAB or AEC for enzymatic detection ? if we need, could...

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Thank you for your email.

The ab98693 is conjugated the HRP enzyme and you will need just the substrate in addition. As a substrate, you can for example use the AEC substrate system ab64252 or the DAB substrate Kit ab64238. The DAB and the AE...

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Following this email,
1.could you tell us the name of substrate please ?
2.which one you recommend for IHC frozen (DAB or AEC) ?

Best regards

Thank you for your email.

As x is not in the office today, I am answering on his behalf.

I can confirm that you can use AEC or DAB depending on your preference and the counterstain of your slides. Both of these substrates are adapte...

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WB with dog heart samples
sees lots of background
10-80 ug protein loaded
block: 5% milk, tried 1h to overnight
Ab: 1/1000, 1/200, 1/20,000 from 1h to overnight
2nd: ab988693, works so so
suggestions to find out which Ab i...

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Thank you for contacting us.
I am sorry to hear that either one or both antibodies are not working as expected.

As we discussed over the phone, the following suggestions will help us to determine if either one of the antibodies or both o...

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We were thinking on your kind advice an after checking your website guidance we finally come to order following antibodies for our IHC-Fr test:
Ab25345 Ab25346 Ab25347 and Ab98693

Before placing order, we highly appreciate your detailed...

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Thank you for contacting us.

1. Yes, a substrate is needed.

2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
> IHC detectionkits 'EXPOSE': http://www.abcam.com/expose (see the 6 first refer...

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1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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