Goat Anti-Mouse IgG1 heavy chain (HRP) (ab97240)


  • 製品名
    Goat Anti-Mouse IgG1 heavy chain (HRP)
    IgG1 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP)
  • 由来種
  • ターゲット生物種
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species.
  • アプリケーション
    適用あり: ICC, ELISA, IHC-P, WBmore details
  • 標識


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 特記事項(標識)
    Molar enzyme/ antibody protein ratio is 4:1
  • ポリ/モノ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab97240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
ELISA 1/10000 - 1/100000.
IHC-P 1/200 - 1/2000.
WB 1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method.


  • IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre


This product has been referenced in:
  • Lianto P  et al. Quail egg homogenate alleviates food allergy induced eosinophilic esophagitis like disease through modulating PAR-2 transduction pathway in peanut sensitized mice. Sci Rep 8:1049 (2018). Read more (PubMed: 29348584) »
  • Hasham MG  et al. Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease. Dis Model Mech 10:259-270 (2017). ELISA . Read more (PubMed: 28250051) »

See all 25 Publications for this product

レビューと Q&A

Western blot
This antibody is super specific! It does not react with mouse IgG2a or human IgG, or any IgG light chain I tested so far.

I use it because I work with mouse tumor lysate in western blot. All tumor lysate contains blood, so IgG is always in the lysates. When my protein of interest is at the same molecular weight of IgG light chain, I use this antibody.

The purpose of western blot I show here is to verify if this antibody reacts with light chain of mouse IgG1 specifically, so there is no primary antibody used.

The antibody is quite clean. The image I show is over-exposed with some background in order to confirm there is absolutely no IgG light chain signal.

Abcam user community

Verified customer

投稿 Oct 14 2015

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these t...

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Thank you for your enquiry.

I can confirm that ab43ERCC1 antibody [3H11] is a mouse monoclonal,IgG2a isotype.

The ab97240 secondary antibody detects mouse IgG1 isotype, so it would not be suitable for use with ab43. You will need ...

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Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. ...

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