Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)

製品の概要

  • 製品名
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed
  • ターゲット生物種
    Mouse
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • アプリケーション
    適用あり: IHC-P, ELISA, WBmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details
  • 免疫原

    Other Immunogen Type corresponding to Mouse IgG.

  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/5000 - 1/50000. For colormetric detection 1/5000 - 1/30000. For chemiluminescent dectection 1/10000 - 1/50000.

画像

  • Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) was used at 1/5000 dilution developed using the ECL technique, with ab86741.

  • IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97040, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

参考文献

This product has been referenced in:
  • Wang B  et al. Brain-derived and glial cell line-derived neurotrophic factor fusion protein immobilization to laminin. Exp Ther Med 13:178-186 (2017). Read more (PubMed: 28123487) »
  • Martínez I  et al. Induction of DNA double-strand breaks and cellular senescence by human respiratory syncytial virus. Virulence 7:427-42 (2016). Read more (PubMed: 26809688) »

See all 17 Publications for this product

レビューと Q&A

Good abtibody

Excellent
Abreviews
Application
Western blot
I use this antibody mostly in WB. I can get good signals(1/10000 dilution).

Upload image condition:
1st antibody ab8245 1/5000 dilution(5% milk)
2nd antibody This antibody 1/10000 dilution(5% milk)
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Abcam user community

Verified customer

投稿 Oct 02 2017

Antibody ab97040 goes through an extra purification step to remove reactivity with immunoglobulins of other species. On the datasheets you will see the following notes in the Purification field.

ab97023 - This antibody was isolated by affini...

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Thank you for contacting us.

Fortunately we offer various protocols and tips that can be found on our webpage under scientific support protocols.

I will give you some links that might be useful for your experiments. However, please n...

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Thank you for confirming that information. I have organised for ab98790 to be sent out to you (Order number xxxxx, Purchase order numberFOCR xxxxx). As we have the Easter bank holidays this will unfortunately only reach you on Wednesday next week.
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Thank you for getting back to me.

I can arrange for a different secondary antibody to be sent to you. In order to organise this I will need the order numbers of ab78021 and ab97040, or the approximate delivery date and delivery address.
...

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Thank you for your enquiry.

Please find attached the MSDS for ab97040, which can also be downloaded from the datasheet of this antibody.

Some of our antibodies are produced in-house and others are sourced from suppliers or academi...

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Again I apologize for this. As you know these replacement vials are typically sent off very quickly. I have requested (again) that this replacement vial be shipped immediately. I have just received a reply from our UK office stating they are now aware ...

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Thanks for your emai. I am sorry you have not received the shipment yet.  I requested a replacement of ab7061 be sent on Dec.1. I am looking into this issue and will let you know when to expect this antibody. Thanks for your patience.

I have requested that a vial of ab7061 be included in the next shipment to your company for this customer. I hope this is helpful. Please contact me again if you have any further questions.

Thank you for contacting us. Please find attached. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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