This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Horseradish Peroxidase (HRP)
The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
ab49969 Immunoprecipitating HA Tag in human 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
Ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.
This product has been referenced in:
Zhang F et al. Ligand Activation of PPAR? by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1a. Theranostics8:610-626 (2018).
Read more (PubMed: 29344293) »
Xia X et al. Lower Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Promotes the Proliferation and Migration of Endometrial Carcinoma. Med Sci Monit23:966-974 (2017).
Read more (PubMed: 28225751) »