製品の概要

  • 製品名
    Goat Anti-Mouse IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
  • ターゲット生物種
    Mouse
  • アプリケーション
    適用あり: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    mouse IgG whole molecule

  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)
    Horseradish Peroxidase (HRP)
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 特記事項
    The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/500 - 1/2500.
IP Use at an assay dependent concentration. PubMed: 17164758
Dot blot Use at an assay dependent concentration.
ELISA 1/100000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2000 - 1/10000. PubMed: 17413034

画像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • ab49969 Immunoprecipitating HA Tag in human 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

  • IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.

参考文献

This product has been referenced in:
  • Zhang GP  et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). WB . Read more (PubMed: 27860183) »
  • Zhang J & Deng X Bupivacaine effectively relieves inflammation-induced pain by suppressing activation of the NF-?B signalling pathway and inhibiting the activation of spinal microglia and astrocytes. Exp Ther Med 13:1074-1080 (2017). WB . Read more (PubMed: 28450945) »

See all 108 Publications for this product

レビューと Q&A

Abreviews
Application
Western blot
Secondary antibody (ab 6789) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for beta actin from pierce antibody.

gel: 10%, 200 V for 35 min.

transfer 17 V for 35 min. using Biorad TURBO
Blocking 5% BSA.
Username

Vicky

Verified customer

投稿 Nov 07 2016

offered free of charge replacement

Thank you for kindly confirming these details. I am sorry these vials of antibody have not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial each of ab6789, ab72439 and ...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion.

I appreciate the time the customer has spent on these experiments, and ...

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Thank you for your response.

I have spoken to my colleague and we can certainly ship the replacement vial directly to your company Qafqazbiotex Co to Georgia. Once you place a new order, we can add this item (free of charge)(ab6789) to your d...

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Thank you for your response and for confirming the order number.

I could arrange a new vial of ab6789 as a free of charge replacement or a credit note. Please could you discuss it with your troubled customer and let me know how you wish to pr...

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Thank you for confirming the batch number.

In my previous e-mail, I have also asked you to provide your Abcam Order number (or your Purchase Order Number) with the date of purchase. We do not have any order in our system from Georgia so it wo...

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Thank you for your enquiry regarding ab6789.

In order to take this complaint further, could you please confirm the batch number and your Abcam Order number (or your Purchase Order Number) with the date of purchase.

As our Abpromise ...

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Thank you very much for your call earlier this month and for your patience.

We now have FITC-conjugated streptavidin available as ab136222-

http://www.abcam.com/Streptavidin-protein-FITC-ab136222.html

I apologize that this...

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Thank you very much for your call yesterday.

As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happ...

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1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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