Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)

製品の概要

  • 製品名Goat Anti-Mouse IgG H&L (FITC) preadsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • アプリケーション適用あり: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • 吸着処理血清
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • 免疫原

    Full length native Mouse IgG (purified).

  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab7064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 15767251
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/1000 - 1/2000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Goat Anti-Mouse IgG H&L (FITC) preadsorbed 画像

  • Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (FITC, pre-adsorbed) (ab7064) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Ab7064 was used at dilution 1/20 with the primary antibody ab7852 in ICC. See the review on ab7852.
  • Ab7064 was used at dilution 1/2000 with the primary antibody ab2378 in IHC-P. See the review on ab2378.

Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064) 使用論文

This product has been referenced in:
  • Harris CJ  et al. Arabidopsis AtMORC4 and AtMORC7 Form Nuclear Bodies and Repress a Large Number of Protein-Coding Genes. PLoS Genet 12:e1005998 (2016). ICC/IF . Read more (PubMed: 27171361) »
  • Morris D  et al. Glutathione synthesis is compromised in erythrocytes from individuals with HIV. Front Pharmacol 5:73 (2014). WB . Read more (PubMed: 24782776) »

See all 3 Publications for this product

Product Wall

Abreviews
Application Immunohistochemistry (Frozen sections)
This is a good antibody and a stable conjugate.
The binding is specific.
The luminous intensity of the conjugate is good and is stable (can still be evaluated after a long time).
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Abcam user community

Verified customer

投稿 Oct 07 2015

Abreviews
Application Flow Cytometry
This is a good functioning antibody with a stable conjugate.
The binding is very specific and the luminous intensity of the conjugate is good.
We have used it in the flow cytometry with a 1/1000 dilution
Username

Abcam user community

Verified customer

投稿 Oct 06 2015

Abreviews
Application Immunocytochemistry/ Immunofluorescence
Mouse Osteoblast cells were grown on cover slips for 48 hrs. Then Cells were fixed with formaldehyde vapor for 10 mins. Cells were permeabilized with Triton-X 100 for 5-7mins. 3% BSA was used for blocking (1hr). 1:100 dilution of mouse anti-talin antibody to mouse was added onto the cells and incubated for 1hr. Then 1:500 dilution of anti-mouse FITC conjugated secondary antibody was added on to the cells and incubated for 1hr. Before proceeding to each step cells were washed with PBS.
Username

Abcam user community

Verified customer

投稿 Jul 05 2013

Thank you for confirming this details.

In my understanding this results show that the ab24590 isfaulty as it has been used with two different secondary antibodies without success. I provide you here therefore with the credit note for this ant...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hearthat the customer is notobtaining a staining with this two antibodies.

The details provided will enable us to investigate this case and will pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"