製品の概要

  • 製品名Goat Anti-Mouse IgG H&L (FITC)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Mouse
  • アプリケーション適用あり: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details
  • 免疫原

    mouse whole molecule

  • 標識FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC (PFA fixed) 1/1000.
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/500 - 1/2500. PubMed: 17989178
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
ICC/IF 1/1000 - 1/5000. (PMID16006199)
ELISA 1/10000 - 1/50000.
WB 1/10000. PubMed: 17217624

Goat Anti-Mouse IgG H&L (FITC) 画像

  • ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.

    See Abreview

  • Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.
  • Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.
  • Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.

Goat Anti-Mouse IgG H&L (FITC) (ab6785) 使用論文

This product has been referenced in:
  • Liu QZ  et al. Identification and isolation of kidney-derived stem cells from transgenic rats with diphtheria toxin-induced kidney damage. Exp Ther Med 12:1651-1656 (2016). Read more (PubMed: 27588086) »
  • Chen Z  et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med 12:1901-1907 (2016). Read more (PubMed: 27588108) »

See all 38 Publications for this product

Product Wall

Thank you very much for your call earlier this month and for your patience.

We now have FITC-conjugated streptavidin available as ab136222-

http://www.abcam.com/Streptavidin-protein-FITC-ab136222.html

I apologize that this...

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Thank you very much for your call yesterday.

As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happ...

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Thank you for your reply and for returning the vial of ab6785.

The vial of ab6717 will be arriving tomorrow on the order ***.


Please let me know if you need anything else, and have a wonderful evening.

Thank you for the follow-up. The article may help, but I think an easy work-around is to try the red channel instead of green, if that is an option. Autofluorescence is generally less of an issue in the red, but you will need a red secondary, such as a...

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Thank you for your call today and for your questions. Please see below for information about our testing discount program, and let me know if you have any further questions about these antibodies or our IHC protocols.

Sinceab63983 has not be...

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Thank you for your inquiry.


We would not recommend ab97234 as a secondary for ab2033 because they do not match in isotype. The secondary ab97234 targets Mouse IgA, but the primary ab2033 is IgG2a.


I would reccomend using...

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Thank you for contacting Abcam. I believe that the staining that you wish to do would work, however by using a mouse primary antibody on mouse tissue, you do run the risk of having high background. I have attached the Abcam protocol for mouse on mouse ...

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Thank you for contacting us. The x-ray film may be able to detect fluorescence emission, but the problem is that the FITC would need to be excited with light to emit light, and that would presumably expose all of the film to the excitation light. Lumin...

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Thank you for contacting Abcam. The product ab6785, the goat polyclonal secondary antibody to mouse IgG - H&L is conjugated with FITC. The only way to detect FITC is with a laser that has the ability to Excite at 494nm and a filter that can detect Emis...

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Thank you for your inquiry. Because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which...

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1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"